Composite

Part:BBa_K3034012:Design

Designed by: Cao Yingying   Group: iGEM19_UESTC-China   (2019-09-16)


A blue-sensitive sterilizing system in Gram-negative bacteria


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 605
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 1297
    Illegal NgoMIV site found at 1590
    Illegal NgoMIV site found at 1684
    Illegal AgeI site found at 319
    Illegal AgeI site found at 1465
    Illegal AgeI site found at 2336
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1354
    Illegal BsaI.rc site found at 218


Design Notes

none


Source

The sequence of blue-sensitive parts was obtained from the part library and the sequence of lysep3-D8 was obtained in the genome of E. coli bacteriophage vB_EcoM-ep3 and Bacillus amyloliquefaciens phage.

References